Journal: Cell

Article Title: A Genetically Defined Compartmentalized Striatal Direct Pathway for Negative Reinforcement

doi: 10.1016/j.cell.2020.08.032

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-MOR Immunostar 24216 Rabbit polyclonal anti-tyrosine hydroxylase Millipore AB152 Chicken polyclonal anti-GFP Aves Labs GFP1020 Rabbit polyclonal anti-RFP Rockland 600-401-379 Rabbit monoclonal anti-HA-Tag Cell Signaling 3724S Mouse monoclonal anti-Parvalbumin Millipore MAB1572 Rabbit polyclonal anti-Somatostatin-14 Peninsula Laboratories T-4103 Goat polyclonal anti-ChAT Millipore AB144P Bacterial and Virus Strains AAV8-Ef1a-fDIO-GCaMP6m Laboratory of Karl Deisseroth N/A AAVdj-hSyn-Cre OFF /Flp ON -hChR2(H134R)-eYFP Fenno et al., 2014 Addgene 55648 AAVdj-hSyn-Cre ON /Flp OFF -hChR2(H134R)-eYFP Fenno et al., 2014 Addgene 55646 AAV8-EF1a-fDIO-Cre-p2A-mCherry Laboratory of Karl Deisseroth N/A AAV5-Ef1a-DIO-hChR2(H134R)-eYFP UNC N/A AAVdj-EF1a-fDIO-eYFP UNC N/A AAV8-hSyn-DIO-KORD-IRES-Mcitrine UNC N/A AAV2/8-Ef1a-fDIO-TVA-mCherry Laboratory of Z. Josh Huang N/A retroAAV2-CBA-fDIO-Cre Vigene Biosciences N/A AAV9-CAGGS-Flex-mKate-T2A-TVA HHMI Janelia Research Campus N/A AAV9-CAGGS-Flex-mKate-T2A-N2c-G HHMI Janelia Research Campus N/A Rbv-CVS-N2c-dG-GFP HHMI Janelia Research Campus Addgene 73461 AAV8-hSyn-DIO-mCherry Addgene Addgene 50459 Experimental Models: Organisms/Strains Mouse: Tg(Drd1a-cre)FK150Gsat/Mmucd (the “D1-Cre” line) MMRRC RRID: MMRRC_029178-UCD Mouse: B6.FVB(Cg)-Tg(Adora2a-cre) KG139Gsat/Mmucd (the “A2A-Cre” line) MMRRC RRID: MMRRC_036158-UCD Mouse: Tshz1-2A-FlpO This study N/A Mouse: Frt-Stop-Frt-TdTomato He et al., 2016 N/A Mouse: Pdyn-IRES-Cre (B6.Cg-129S-Pdyn tm1.1(cre)Mjkr/LowlJ) The Jackson Laboratory JAX: 027958 Mouse: Ai14 (B6.Cg-Gt(ROSA) 26Sortm14(CAG-tdTomato)Hze/J) The Jackson Laboratory JAX: 007908 Software and Algorithms ImageJ (Fiji) software NIH https://fiji.sc/ MATLAB Mathworks https://www.mathworks.com/ GraphPad Prism 7 GraphPad Software https://www.graphpad.com/ Open in a separate window KEY RESOURCES TABLE Tshz1 labels a population of striatal direct pathway medium spiny neurons (dMSNs) Tshz1 -expressing ( Tshz1 + ) dMSNs are localized in the striosome Tshz1 + striosomal dMSNs represent punishment and drive negative reinforcement Pdyn labels another population of striosomal dMSNs mediating positive reinforcement

Techniques: Software

Journal: Cell reports

Article Title: Food-induced dopamine signaling in AgRP neurons promotes feeding

doi: 10.1016/j.celrep.2022.111718

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: AAV8-hSyn-DIO-hM3Dq-mCherry (Addgene 44,361-AAV8; 9 × 10^12 viral genomes/mL), AAV8-hSyn-DIO-mCherry (Addgene 50,459-AAV8; 1.2 × 10^13 viral genomes/mL), AAV2-Ef1a-DIO-hChR2(H134R)-EYFP-WPRE-pA (University of North Carolina at Chapel Hill Gene Therapy Center Vector Core Services; 2.1 × 10^13 viral genomes/mL), AAV1-hSyn-FRT-TeNT-GFP (generated and produced in the Zweifel lab; 10^12 viral genomes/mL), AAV1-Ef1a-ConFon-hM3-HA (ssAAV-1/2-hEf1alpha/hTLV1-Fon/Con [dFRT-HA_hM3D(Gq) (rev)-I-dlox-I-HA_hM3D(Gq)-I-dlox-I-HA_hM3D(Gq) (rev)-dFRT]-WPRE-hGHp(A); entry v605 at https://vvf.ethz.ch ; 4.6 × 10^12 viral genomes/mL), AAV1-Ef1a-ConFoff-hM3-HA (generated in the Güler lab, produced at the University of North Carolina at Chapel Hill Gene Therapy Center Vector Core Services; 2.2 × 10^13 viral genomes/mL), AAV8-hSyn-Con/Fon-ChR2-EYFP (Addgene 55,645-AAV8; 2.1 × 10^13 viral genomes/mL), retrograde AAV-hSyn-DIO-hM3Dq-mCherry (Addgene 44361-AAVrg; 7 × 10^12 viral genomes/mL), AAV9-hSyn-GRAB-DA2h (Addgene 140,554-AAV9; 2.5 × 10^13 viral genomes/mL) were injected to the ARC (ML: ± 0.30 mm, AP: − 1.40 mm, DV: −5.90 mm).

Techniques: Virus, Plasmid Preparation, Recombinant, RNAscope, Multiplex Assay, Software